Recombinant mNeonGreen 抗体 (DBCO)
适用: Branchiostoma lanceolatum
ICC, IF
宿主: Alpaca
Monoclonal
1E2
DBCO
single-domain Antibody (sdAb)
Recombinant Antibody
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- 抗原
- mNeonGreen
- 抗体类型
- Recombinant Antibody
- 片段
- single-domain Antibody (sdAb)
- 适用
- Branchiostoma lanceolatum
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宿主
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Alpaca
- Expression System
- E.coli
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克隆类型
- 单克隆
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标记
- DBCO
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应用范围
- Immunocytochemistry (ICC), Immunofluorescence (IF)
- 特异性
- Recognizes mNeonGreen. Does not cross-react with any GFP- or dsRed, of TagBFP derivatives.
- 纯化方法
- Produced in: E.coli
- 免疫原
- Our nanobody binds specifically and strongly to mNeonGreen and possibly to its predecessor
- 克隆位点
- 1E2
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- 应用备注
- Western Blotting not recommended. The sdAbs tend to recognize native protein conformation only.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconsitute with 250 µL 50% glycerol for storage at -80 °C
- 缓冲液
- sdAbs are lyophilized from PBS pH 7.4.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Vials containing lyophilized protein can be stored at 4°C for up to 6 months. Vials containing reconstituted protein should be stored at -20°C or below.
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- 抗原
- mNeonGreen
- 背景
- MNeonGreen is a very bright monomeric green fluorescent protein that matures relatively fast when expressed as a fluorescent probe in cells. For this reason, since its discovery in 2013, mNeonGreen has gained major attention among biological sciences. It is a synthetically derived version of the wild-type protein found in Branchiostoma lanceolatum.
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