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Recombinant GFP 抗体 (AA 1-238)

The 小鸡 Chimeric anti-GFP antibody is suitable to detect GFP in samples from Jellyfish. It has been validated for WB, ELISA, IHC 和 ICC.
产品编号 ABIN7830377
发货至: 中国
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Our Local Distributor

中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant GFP 抗体 (AA 1-238) (ABIN7830377)

抗原

See all GFP 抗体
GFP (Green Fluorescent Protein (GFP))

抗体类型

Recombinant Antibody

适用

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Jellyfish

宿主

  • 115
  • 93
  • 38
  • 18
  • 16
  • 4
  • 4
  • 2
小鸡, 小鼠

克隆类型

  • 152
  • 125
  • 5
Chimeric

标记

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  • 1
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  • 1
This GFP antibody is un-conjugated

应用范围

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

质量等级

Carrier-free

克隆位点

N86-38
  • 抗原表位

    • 35
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    • 10
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    AA 1-238

    Expression System

    Mammalian cells

    原理

    Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb (BSA and Azide free)

    交叉反应 (详细)

    Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)

    产品特性

    This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.

    纯化方法

    Purified by affinity chromatography

    免疫原

    Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli

    亚型

    IgY
  • 应用备注

    1:500-1:3000

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    PBS pH 7.4

    储存液

    Azide free

    储存条件

    4 °C,-20 °C

    储存方法

    Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

    有效期

    24 months
  • 抗原

    GFP (Green Fluorescent Protein (GFP))

    别名

    GFP

    背景

    Green fluorescent protein

    Our chicken Anti-GFP carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.

    GFP

    分子量

    27 kDa

    UniProt

    P42212
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