Recombinant GFP 抗体 (AA 1-238)
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北京 101111
Quick Overview for Recombinant GFP 抗体 (AA 1-238) (ABIN7830377)
抗原
See all GFP 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
质量等级
克隆位点
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抗原表位
- AA 1-238
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Expression System
- Mammalian cells
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原理
- Anti-GFP (N86/38) Recombinant Chicken Chimeric mAb (BSA and Azide free)
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交叉反应 (详细)
- Cross-reacts with Cyan and Yellow fluorescent protein (CFP and YFP, respectively)Does not cross-react with Red fluorescent protein (dsRed)
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产品特性
- This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone N86/38 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone N86/38 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media and buffer exchanged into 1X PBS.
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纯化方法
- Purified by affinity chromatography
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免疫原
- Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein (accession number P42212) produced recombinantly in E. Coli
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亚型
- IgY
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应用备注
- 1:500-1:3000
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS pH 7.4
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储存液
- Azide free
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储存条件
- 4 °C,-20 °C
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储存方法
- Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
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有效期
- 24 months
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- GFP (Green Fluorescent Protein (GFP))
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别名
- GFP
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背景
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Green fluorescent protein
Our chicken Anti-GFP carrier-free recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone N86/38. It detects n/a GFP and is purified by affinity chromatography. It works in WB, IHC, ICC, ELISA.
GFP
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分子量
- 27 kDa
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UniProt
- P42212
抗原
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