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Recombinant Histone H2B 抗体

The 兔 单克隆 anti-Histone H2B antibody (Clone A831) (ABIN7828263) specifically detects Histone H2B in WB 和 IHC. The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7828263
发货至: 中国
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Quick Overview for Recombinant Histone H2B 抗体 (ABIN7828263)

抗原

See all Histone H2B 抗体
Histone H2B

抗体类型

Recombinant Antibody

适用

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人, 小鼠, 大鼠

宿主

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克隆类型

  • 218
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单克隆

标记

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This Histone H2B antibody is un-conjugated

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC)

克隆位点

A831
  • 原理

    Recombinant Histone H2B (Ubiquityl Lys120) Monoclonal Antibody

    纯化方法

    Protein A purified

    免疫原

    Ubiquitinated human histone H2B (Lys120) peptide

    亚型

    IgG, kappa
  • 应用备注

    WB 1:500-1:2000,IHC 1:200-1:1000

    限制

    仅限研究用
  • 浓度

    1 mg/mL

    缓冲液

    PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 % protein protectant.

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

    有效期

    12 months
  • 抗原

    Histone H2B

    背景

    HIST1H2BG,H2B.1A,H2B/a,H2BFA,H2BFL,HIST1H2BC,H2BFH,HIST1H2BE,H2BFG,HIST1H2BF,H2BFK,HIST1H2BI,H2BC4,H2BC6,H2BC7,H2BC8,H2BC10,Ubiquitin (Ub) is a highly conserved 76-amino acid protein that plays a critical role in regulating a wide range of cellular processes. Through a three-step process involving Ub-activating (E1), Ub-conjugating (E2), and Ub-ligating (E3) enzymes, ubiquitin covalently attaches to target proteins and marks them for proteasomal degradation, alters protein-protein interactions, modulates membrane protein trafficking, and controls the activity of many signal transduction pathways. Mono-ubiquitination of Histone H2B at Lys120 (H2BK120ub) serves signaling purposes and is reversible by ubiquitin-specific proteases (USPs). The enzymes responsible for H2BK120ub are Rad6 E2 proteins (HR6A and HR6B in human) and Bre1 E3 ligase (RNF20 and RNF40 in human). This modification triggers a ""trans-histone"" modification cascade, resulting in trimethylation of Lys4 and Lys79 in histone H3, which promotes gene activity. Cat.No. Product Name Clone No. IF:{{item.impact}} Journal:{{item.journal}} ({{item.year}}) DOI:{{item.doi}} Reactivity:{{item.species}} Sample Type:{{item.sample_type}} Previous {{ page }} Next Q{{(FAQpage.currentPage - 1)*pageSize+index+1}}:{{item.name}} Previous {{ page }} Next [

    分子量

    Calculated MW: 23 kDa

    Observed MW: 23 kDa

    UniProt

    P62807
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