Recombinant HMGB1 抗体 (N-Term)
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Quick Overview for Recombinant HMGB1 抗体 (N-Term) (ABIN7826123)
抗原
See all HMGB1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- N-Term
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原理
- HMGB1 antibody (HMV317)
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免疫原
- Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human HMGB1. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Colon: A strong HMGB1 positivity should be seen in all cell types.
Product Description/Background: HMGB1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Testis: Spermatids and more mature spermatocytes should be HMGB1 negative.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- HMGB1 (High Mobility Group Box 1 (HMGB1))
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别名
- HMGB1
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背景
- Gene Alias: high mobility group box 1,HMG-1,HMG1,HMG3,SBP-1
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UniProt
- P09429
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途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
抗原
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