Recombinant Claudin 3 抗体 (Intracellular)
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北京 101111
Quick Overview for Recombinant Claudin 3 抗体 (Intracellular) (ABIN7826112)
抗原
See all Claudin 3 (CLDN3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Intracellular
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原理
- Claudin 3 antibody (HMV309)
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免疫原
- Synthetic peptide encompassing a sequence within the Intracellular domain of human Claudin 3. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Liver: A strong membranous CLDN3 staining should be seen in bile ducts. In addition, a weak to moderate membranous CLDN3 staining should be seen in hepatocytes (often seen predominantly at the bile secreting pole).
Product Description/Background: Claudin 3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Stomach: Most epithelial cells should be CLDN3 negative, while a strong membranous CLDN3 staining of a small subset of epithelial cells in the neck and in glandular pits must be seen.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- Claudin 3 (CLDN3)
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别名
- Claudin 3
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背景
- Gene Alias: claudin 3,C7orf1,CPE-R2,CPETR2,HRVP1,RVP1
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UniProt
- O15551
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途径
- Hepatitis C
抗原
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