Recombinant Caspase 3 抗体 (Center)
Quick Overview for Recombinant Caspase 3 抗体 (Center) (ABIN7826109)
抗原
See all Caspase 3 (CASP3) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Center
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原理
- Caspase-3 antibody (HMV307)
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免疫原
- Recombinant protein encompassing a sequence within the center region of human Caspase 3. The exact sequence is proprietary.
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亚型
- IgG
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应用备注
- IHC 1:100-1:200
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说明
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Positive Control: Stomach: At least a moderate caspase-3 positivity should be seen in surface epithelial cells.
Product Description/Background: Caspase-3 antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Stomach: Deep gastric glands and muscular cells must be caspase-3 negative.
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实验流程
- Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.
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限制
- 仅限研究用
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状态
- Liquid
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
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有效期
- 24 months
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- Caspase 3 (CASP3)
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别名
- Caspase-3
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背景
- Gene Alias: caspase 3,CPP32,CPP32B,SCA-1
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UniProt
- P42574
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途径
- Apoptosis, Caspase Cascade in Apoptosis, Sensory Perception of Sound, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Activated T Cell Proliferation
抗原
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