MCM4 抗体 (AA 343-659)
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北京 101111
Quick Overview for MCM4 抗体 (AA 343-659) (ABIN7825548)
抗原
See all MCM4 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 343-659
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原理
- Anti-Zebrafish MCM4 Antibody
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产品特性
- Anti-Zebrafish MCM4 Antibody catalog #AZQ6NZV2. Tested in WB applications. This antibody reacts with Zebrafish. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived zebrafish MCM4 recombinant protein (Position: M343-I659)
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Zebrafish
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))
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别名
- MCM4
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背景
- MCM4(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 4), also called CDC21, S. POMBE, HOMOLOG OF, is a protein that in humans is encoded by the MCM4 gene. MCM4 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM4 gene is mapped to 8q11.21. The 864-amino acid MCM4 protein has an observed molecular mass of 97 kD by SDS-PAGE, which is similar to its calculated molecular mass of 96.6 kD. Western blot analysis with and without phosphatase treatment suggested that MCM4 is highly phosphorylated in mitotic cells. In the absence of DDK, CDK phosphorylation at the distal part of the Mcm4 NSD becomes crucial.MCM4 encodes a subunit of the MCM2-7 complex(also known as MCM2-MCM7), the replication licensing factor and presumptive replicative helicase.
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途径
- DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA
抗原
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