EIF2A 抗体 (AA 24-585)
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北京 101111
Quick Overview for EIF2A 抗体 (AA 24-585) (ABIN7825362)
抗原
See all EIF2A 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 24-585
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原理
- Anti-EIF2A Antibody
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交叉反应 (详细)
- No cross-reactivity with other proteins
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产品特性
- Anti-EIF2A Antibody catalog # A02418-1. Tested in WB, IHC, Flow Cytometry, ELISA applications. This antibody reacts with Human, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human EIF2A recombinant protein (Position: H24-I585). Human EIF2A shares 92.5% amino acid (aa) sequence identity with mouse EIF2A.
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亚型
- IgG
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应用备注
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Western blot, 0.25-0.5 μg/mL, Human, Rat
Immunohistochemistry, 2-5 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/mL
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Adding 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
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储存条件
- 4 °C,-20 °C
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储存方法
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At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
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- EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))
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别名
- EIF2A
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背景
- This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.
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分子量
- 65 kDa
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基因ID
- 83939
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途径
- Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly
抗原
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