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SKP2 抗体 (AA 351-424)

Name: SKP2 抗体 (AA 351-424)
SKU: ABIN737601
Availability: OutOfStock

This anti-SKP2 antibody is a 兔多克隆 antibody detecting SKP2 in WB, ELISA, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated (ABIN737601) at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SKP2 抗体 (AA 351-424))

IHC

MCF-7 cells were stained with SKP2 Polyclonal Antibody, Unconjugated at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. (SKP2 抗体 (AA 351-424))

IF (cc)

293T lysates probed with SKP2 Polyclonal Antibody, Unconjugated at 1:500 dilution and 4˚C overnight incubation. (SKP2 抗体 (AA 351-424))

Formalin-fixed and paraffin embedded human thyroid carcinoma labeled with Anti-SKP2/skp2 p45 Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining. (SKP2 抗体 (AA 351-424))

IHC (p)

Hela cells probed with SKP2 Polyclonal Antibody, Unconjugated (green) at 1:100 for 30 minutes followed by a PE conjugated secondary antibody compared to unstained cells (blue), secondary only (light blue), and isotype control (orange). (SKP2 抗体 (AA 351-424))

FACS

5 images

(4 references)

产品编号 ABIN737601

发货至: 中国

Quick Overview for SKP2 抗体 (AA 351-424) (ABIN737601)

抗原

See all SKP2 抗体

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This SKP2 antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 351-424

交叉反应

人, 小鼠, 大鼠

预测反应

Cow,Rabbit

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human Skp2

亚型

IgG
anti-S-Phase Kinase-Associated Protein 2 (SKP2) (C-Term) antibody
SKP2 适用: 人, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-100) antibody
SKP2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 WB, IHC 宿主: 兔 Monoclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 43-397) antibody
SKP2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 ELISA, IHC, IF 宿主: 兔 Monoclonal 6D4 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 ELISA, IF, FACS 宿主: 兔 Monoclonal 3C11 unconjugated Recombinant Antibody

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (AA 1-424) antibody
SKP2 适用: 人 WB, PLA 宿主: 小鼠 Polyclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) antibody
SKP2 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-S-Phase Kinase-Associated Protein 2 (SKP2) (N-Term) antibody
SKP2 适用: 人, 小鼠, 大鼠 WB, IHC, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Pan, Li, Zhong, Chen, Chen, Gao, Wu, Guo: "RIG-I inhibits pancreatic β cell proliferation through competitive binding of activated Src." in: Scientific reports, Vol. 6, pp. 28914, (2016) (PubMed).

Yang, Wang, Wang, Zhang, Zhang, Lu, Wang: "mTOR is involved in 17?-estradiol-induced, cultured immature boar Sertoli cell proliferation via regulating the expression of SKP2, CCND1, and CCNE1." in: Molecular reproduction and development, Vol. 82, Issue 4, pp. 305-14, (2015) (PubMed).

Jiao, Yi, Rong, Kee, Zhong: "MicroRNA-1285 regulates 17?-estradiol-inhibited immature boar Sertoli cell proliferation via adenosine monophosphate-activated protein kinase activation." in: Endocrinology, pp. en20141982, (2015) (PubMed).

Sun, Yang, Luo, Wang, Chen, Zhang, Wang, Li: "Thyroid hormone inhibits the proliferation of piglet Sertoli cell via PI3K signaling pathway." in: Theriogenology, (2014) (PubMed).
抗原

SKP2 (S-Phase Kinase-Associated Protein 2 (SKP2))

别名

SKP2

背景

Synonyms: p45, FBL1, FLB1, FBXL1, S-phase kinase-associated protein 2, Cyclin-A/CDK2-associated protein p45, F-box protein Skp2, F-box/LRR-repeat protein 1, p45skp2, SKP2
Background: Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, FOXO1, UBP43, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1. Recognizes CDKN1A in association with CCNE1 or CCNE2 and CDK2. Promotes ubiquitination and destruction of CDH1 in a CK1-Dependent Manner, thereby regulating cell migration.

基因ID

6502

UniProt

Q13309

途径

Mitotic G1-G1/S Phases