VLDLR 抗体 (C-Term) (CF®740)
Quick Overview for VLDLR 抗体 (C-Term) (CF®740) (ABIN7661870)
抗原
See all VLDLR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- C-Term
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原理
- VLDL-Receptor (Very Low Density Lipoprotein Receptor) (VLDLR/1337), CF740 conjugate
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产品特性
- VLDLR (very low density lipoprotein receptor) is a member of the LDL receptor gene family, which includes LDL receptor, LRP, megalin, VLDLR and ApoER2. The LDL receptor family is characterized by a cluster of cysteine-rich class A repeats, epidermal growth factor (EGF)-like repeats, YWTD repeats and an O-linked sugar domain. VLDLR associates with RAP (receptor associated protein) during receptor folding, and RAP facilitates the secretion of the extracellular region of VLDLR. VLDLR is thought to mediate the interaction of extracellular Reelin and cytosolic mDab1 (mammalian disabled protein), which activates a tyrosine kinase. This pathway regulates the migration of neurons along the radial glial fiber network during brain development.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human VLDLR fragment from c-terminal
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亚型
- IgG1
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin) 1-2 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HeLa or U-251 cells. Pancreas, Cerebellum, Heart or Skeletal Muscle.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- VLDLR (Very Low Density Lipoprotein Receptor (VLDLR))
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别名
- VLDL Receptor
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背景
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Synonyms: Very low-density lipoprotein receptor, VLDL receptor, VLDL-R, VLDLR
Gene Symbol: VLDLR
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分子量
- 143-161 kDa
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基因ID
- 7436
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途径
- Cellular Response to Molecule of Bacterial Origin
抗原
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