VEGF 抗体 (CF®594)
Quick Overview for VEGF 抗体 (CF®594) (ABIN7661846)
抗原
See all VEGF 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- VEGF (Vascular Endothelial Growth Factor)(VG76e), CF594 conjugate
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产品特性
- This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human VEGF189 recombinant protein
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亚型
- IgG1, kappa
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应用备注
- ELISA: For coating, order Ab without BSA, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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说明
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Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- VEGF (Vascular Endothelial Growth Factor (VEGF))
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别名
- VEGF
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背景
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Synonyms: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA
Gene Symbol: VEGFA
Tissue Expression: Endothelial cells
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分子量
- 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)
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基因ID
- 7422
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UniProt
- P15692
抗原
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