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VEGF 抗体 (CF®740)

This CF®740-conjugated 小鼠 单克隆 anti-VEGF antibody (Clone VG76e) specifically detects VEGF in WB, IHC, IHC (fp) 和 Func. The antibody is reactive with 人 samples.
产品编号 ABIN7661842
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for VEGF 抗体 (CF®740) (ABIN7661842)

抗原

See all VEGF 抗体
VEGF (Vascular Endothelial Growth Factor (VEGF))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This VEGF antibody is conjugated to CF®740

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Functional Studies (Func)

克隆位点

VG76e
  • 原理

    VEGF (Vascular Endothelial Growth Factor)(VG76e), CF740 conjugate

    产品特性

    This MAb recognizes proteins of 19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing), identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). It is highly specific to VEGF, which is a homodimeric, disulfide-linked glycoprotein with a close homology to platelet-derived growth factor (PDGF). There are multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis, which promotes tumor progression and metastasis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Human VEGF189 recombinant protein

    亚型

    IgG1, kappa
  • 应用备注

    ELISA: For coating, order Ab without BSA, Immunofluorescence: 1-2 μg/mL, Optimal dilution for a specific application should be determined.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody

    说明

    Positive Control: Tumor cells in astrocytomas, breast or ovarian carcinomas.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    VEGF (Vascular Endothelial Growth Factor (VEGF))

    别名

    VEGF

    背景

    Synonyms: MVCD1, Vascular Endothelial Growth Factor A (VEGF-A), Vascular Permeability Factor (VPF), VEGFA

    Gene Symbol: VEGFA

    Tissue Expression: Endothelial cells

    分子量

    19-22 kDa (reducing) and 38 kDa-44 kDa (non-reducing)

    基因ID

    7422

    UniProt

    P15692
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