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UCHL1 抗体 (CF®740)

This anti-UCHL1 antibody is a 小鼠 单克隆 antibody detecting UCHL1 in WB, IHC, IF, FACS 和 IHC (fp). Suitable for 人, 大鼠, 小鼠, Cow 和 Pig.
产品编号 ABIN7661602
发货至: 中国

Quick Overview for UCHL1 抗体 (CF®740) (ABIN7661602)

抗原

See all UCHL1 抗体
UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

适用

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人, 大鼠, 小鼠, Cow, Pig

宿主

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小鼠

克隆类型

  • 104
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单克隆

标记

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This UCHL1 antibody is conjugated to CF®740

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

31A3
  • 原理

    pgp9.5 (UCHL-1)(31A3), CF740 conjugate

    产品特性

    This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Native UchL1 (PGP9.5) protein from brain

    亚型

    IgG1, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Cerebellum

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

    别名

    PGP9.5

    背景

    Synonyms: Gracile Axonal Dystrophy|Neuron Cytoplasmic Protein 9.5|Park5|Parkinson Disease 5|PGP95|Protein Gene Product 9.5|Ubiquitin Carboxyl-terminal Esterase L1|Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1|Ubiquitin Thioesterase L1|Ubiquitin Thiolesterase L1

    Gene Symbol: UCHL1

    Tissue Expression: Neurons

    分子量

    20-30 kDa

    基因ID

    7345

    UniProt

    P09936

    途径

    Feeding Behaviour
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