Recombinant UCHL1 抗体 (CF®405S)
Quick Overview for Recombinant UCHL1 抗体 (CF®405S) (ABIN7661598)
抗原
See all UCHL1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
 - PGP9.5 / UchL1 (pan-Neuronal Marker) (rUCHL1/775), CF405S conjugate
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产品特性
 - This MAb reacts with a protein of 20-30 kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
 - Recombinant full-length human UCHL1 protein
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亚型
 - IgG1, kappa
 
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应用备注
 - Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 1-2 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Cerebellum
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.1 mg/mL
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缓冲液
 - PBS, 0.1 % BSA, 0.05 % azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Protect from light
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储存条件
 - 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
 - 24 months
 
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- UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))
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别名
 - PGP9.5
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背景
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Synonyms: Gracile Axonal Dystrophy, Neuron Cytoplasmic Protein 9.5, Park5, Parkinson Disease 5, PGP95, Protein Gene Product 9.5, Ubiquitin Carboxyl-terminal Esterase L1, Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1, Ubiquitin Thioesterase L1, Ubiquitin Thiolesterase L1
Gene Symbol: UCHL1
Tissue Expression: Neurons
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分子量
 - 20-30 kDa
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基因ID
 - 7345
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UniProt
 - P09936
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途径
 - Feeding Behaviour
 
抗原
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