Recombinant TYR 抗体 (CF®647)
Our Local Distributor
北京 101111
Quick Overview for Recombinant TYR 抗体 (CF®647) (ABIN7661464)
抗原
See all TYR 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
-
-
原理
- Tyrosinase (Melanoma Marker) (TYR/2024R), CF647 conjugate
-
产品特性
- This antibody recognizes a cluster of proteins between 70-80 kDa, identified as tyrosinase. Occasionally a minor band at 55 kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
-
免疫原
- Recombinant full-length human tyrosinase (TYR) protein
-
亚型
- IgG
-
-
anti-Tyrosinase (TYR) (AA 20-340) antibody
TYR 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 1-377) antibodyTYR 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A2-F4 unconjugated
anti-Tyrosinase (TYR) (AA 486-513) antibodyTYR 适用: 人 WB, IHC, IF, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人, 小鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 1-377) antibodyTYR 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal T311 unconjugated
anti-Tyrosinase (TYR) (AA 486-513), (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19155 unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal T311 unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
-
-
应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA pH 9.0 or 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
-
说明
-
Positive Control: SK-MEL-19, SK-MEL-30 cells or Melanoma.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.1 mg/mL
-
缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
-
储存液
- Sodium azide
-
注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
注意事项
- Protect from light
-
储存条件
- 4 °C
-
储存方法
-
Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
-
有效期
- 24 months
-
-
- TYR (Tyrosinase (TYR))
-
别名
- Tyrosinase
-
背景
-
Synonyms: ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR
Gene Symbol: TYR
Tissue Expression: Melanocytes
-
分子量
- 70-80 kDa
-
基因ID
- 7299
-
UniProt
- P14679
抗原
-
