TYR 抗体 (AA 233-247)
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北京 101111
Quick Overview for TYR 抗体 (AA 233-247) (ABIN3024010)
抗原
See all TYR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 233-247
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原理
- Tyrosinase Antibody
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序列
- IPYWDWRDAE KCDIC
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纯化方法
- Protein G affinity chromatography
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免疫原
- Recombinant Tyrosinase protein was used as the immunogen for this antibody. Subsequent testing has localized the epitope to within amino acids 233-247 (IPYWDWRDAEKCDIC) (see Citation 12).
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亚型
- IgG2a, kappa
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anti-Tyrosinase (TYR) (AA 1-377) antibody
TYR 适用: 人 WB, IF, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 2A2-F4 unconjugated
anti-Tyrosinase (TYR) (AA 20-340) antibodyTYR 适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 486-513) antibodyTYR 适用: 人 IHC, WB, IF, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (AA 1-377) antibodyTYR 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人, 小鼠, 猴 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人, 小鼠, 大鼠 IHC, WB, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) antibodyTYR 适用: 人 IHC, IF, FACS 宿主: 小鼠 Monoclonal T311 unconjugated
anti-Tyrosinase (TYR) (AA 486-513), (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB19155 unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-Tyrosinase (TYR) (C-Term) antibodyTYR 适用: 人, 猴 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C
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储存方法
- Store the Tyrosinase antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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- TYR (Tyrosinase (TYR))
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别名
- Tyrosinase
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背景
- Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway, the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients.
抗原
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