TNC 抗体 (CF®740)
Quick Overview for TNC 抗体 (CF®740) (ABIN7660670)
抗原
See all TNC 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Tenascin(T2H5), CF740 conjugate
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产品特性
- In Western blotting, this antibody reacts with two bands of ~MW of 210 kDa and 300 kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neo-vasculature and stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces and increases markedly in hyper-proliferative diseases and in stroma of many neoplasms, including gliomas, breast, squamous and lung carcinomas.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human breast carcinoma
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亚型
- IgG1, kappa
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anti-Tenascin C (TNC) (AA 513-621) antibody
TNC 适用: 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tenascin C (TNC) antibodyTNC 适用: 人 WB, IP, IHC (p) 宿主: 小鼠 Monoclonal T2H5 unconjugated
anti-Tenascin C (TNC) (AA 49-181) antibodyTNC 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Tenascin C (TNC) (AA 175-342) antibodyTNC 适用: 大鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Tenascin C (TNC) (AA 1001-1100) antibodyTNC 适用: 大鼠, 小鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tenascin C (TNC) (AA 49-181) antibodyTNC 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tenascin C (TNC) (AA 181-290) antibodyTNC 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Tenascin C (TNC) (AA 174-621) antibodyTNC 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tenascin C (TNC) antibodyTNC 适用: 人 IHC, StM 宿主: 兔 Monoclonal TNC-2981R unconjugated Recombinant Antibody
anti-Tenascin C (TNC) antibodyTNC 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal TNC-3635 unconjugated Recombinant Antibody
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with rat, others not known. Immunohistology formalin-fixed 2-4 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Extracellular matrix in tonsil and blood vessels. Stroma of many tumors such as breast, squamous cell, and lung carcinomas. Staining of normal fibroblasts serves as internal positive control
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- TNC (Tenascin C (TNC))
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别名
- Tenascin
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背景
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Synonyms: Cytotactin, Glioma-associated-extracellular matrix antigen, GMEM, GP 150-225, Hexabrachion (HXB), JI, Myotendinous antigen, Neuronectin, TNC
Gene Symbol: TNC
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分子量
- 210 kDa and 300 kDa
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基因ID
- 3371
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UniProt
- P24821
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途径
- Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development
抗原
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