PLK1 抗体 (CF®647)
Quick Overview for PLK1 抗体 (CF®647) (ABIN7658759)
抗原
See all PLK1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PLK1 (Marker of Mitosis)(AZ44), CF647 conjugate
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产品特性
- Plk (for polo-like kinase) encodes a serine/threonine kinase that is closely related to polo and CDC5, genes that are required for passage through mitosis in Drosophila and Saccharomyces, respectively. Polo and Cdc5 both code for proteins that are involved in regulating the function of the mitotic spindle. Plk protein accumulates in the cell during the S and G2 phases of the cell cycle, Plk protein content and catalytic activity peak at the onset of mitosis, followed by a rapid reduction after mitosis. Plk expression is detectable in mitotically active tissues such as colon and placenta, as well as in tumors of various origins. It has also been suggested that Plk may serve as a marker of cell proliferation. Required for recovery after DNA damage checkpoint and entry into mitosis. Required for kinetochore localization of BUB1B. Phosphorylates SGOL1. Required for spindle pole localization of isoform 3 of SGOL1 and plays a role in regulating its centriole cohesion function. Phosphorylates BORA, and thereby promotes the degradation of BORA. Contributes to the regulation of AURKA function. Regulates TP53 stability through phosphorylation of TOPORS. Useful for confirming that extracts have maintained CSF status and have not yet been activated into interphase. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length PLK1 Xenopus laevis protein
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亚型
- IgG1, kappa
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anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibody
PLK1 适用: 人, 小鼠 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 3C11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4G11 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 1-603) antibodyPLK1 适用: 人 WB, ELISA, IF, IP, PLA 宿主: 小鼠 Monoclonal 2G12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 5C1 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 6C6 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal 1D4 unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人, 犬, 猴 WB, IF, IP, FACS 宿主: 小鼠 Monoclonal 8C12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 201-300) antibodyPLK1 适用: 人 WB, ELISA, IHC (p), IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Polo-Like Kinase 1 (PLK1) antibodyPLK1 适用: 人 WB, IHC, IP, IHC (p), FACS 宿主: 小鼠 Monoclonal 3F12 unconjugated
anti-Polo-Like Kinase 1 (PLK1) (AA 331-508) antibodyPLK1 适用: 人 ELISA, ICC 宿主: 小鼠 Monoclonal 1D1 unconjugated
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应用备注
- Does not react with Human or Mouse, ELISA: For coating, order antibody without BSA, Western blot 1-2 μg/mL, Optimal dilution for a specific application should be determined by user.. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody
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说明
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Positive Control: Mitotic CSF Xenopus egg extract.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- PLK1 (Polo-Like Kinase 1 (PLK1))
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别名
- PLK1
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背景
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Synonyms: Cell cycle regulated protein kinase, Polo like kinase 1 (PLK1), Serine/threonine-protein kinase PLK1, STPK13
Gene Symbol: plk1.S
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分子量
- 66 kDa
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基因ID
- 380481
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UniProt
- P70032
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途径
- Cell Division Cycle, M Phase
抗原
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