Plasma Cell Marker 抗体 (CF®740)
This anti- antibody is a 小鼠 单克隆 antibody detecting in IHC, IHC (fp) 和 IF. Suitable for 人.
Quick Overview for Plasma Cell Marker 抗体 (CF®740) (ABIN7658624)
抗原
Plasma Cell Marker
适用
人
宿主
小鼠
克隆类型
单克隆
标记
CF®740
应用范围
Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunofluorescence (IF)
克隆位点
LIV3G11
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原理
- Plasma Cell Marker(LIV3G11, same as 7B18), CF740 conjugate
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产品特性
- This antibody recognizes an intra-cytoplasmic antigen, which shows a very high degree of specificity for plasma cells. This antigen is present in normal as well as neoplastic plasma cells. Plasma cells, which are large lymphocytes derived from an antigen-specific B cell, secrete antibodies and are responsible for humoral immunity. Plasma cells differentiate from B cells upon stimulation by CD4 lymphocytes. The B cell acts as an antigen-presenting cell (APC), consuming an offending pathogen, which is taken up by the B cell by phagocytosis and broken down within proteosomes. Plasma cells contain basophilic cytoplasm, their nucleus contains heterochromatin organized in a characteristic cartwheel arrangement. This MAb superbly recognizes normal and neoplastic plasma cells in routine formalin-fixed, paraffin-embedded tissue sections. It is of potential value in identifying myeloma or plasmacytoma in bone marrow or other tissues. It also helps differentiate lympho-plasmacytoid lymphoma from lymphocytic and follicular lymphoma. Note that this MAb is not suitable for staining frozen tissues. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Pancreatic cancer-related mucin
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亚型
- IgG2a
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anti-Plasma Cell Marker antibody
适用: 人 IHC, StM 宿主: 小鼠 Monoclonal LIV3G11-same as 7B18 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Does not react with rat, others not known. Immunohistology formalin-fixed 0.1-0.2 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Tonsil or lymph node
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Plasma Cell Marker
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背景
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Synonyms: Plasma B cell, Plasmocytes
Tissue Expression: B-cells|Lymphocytes
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分子量
- Not Known
抗原
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