PLAP 抗体 (CF®740)
Quick Overview for PLAP 抗体 (CF®740) (ABIN7658620)
抗原
See all PLAP (ALPP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- PLAP (Placental Alkaline Phosphatase)(ALP/870), CF740 conjugate
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产品特性
- Reacts with a 70 kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human ALPP protein
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亚型
- IgG2b, kappa
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anti-Placental Alkaline Phosphatase (ALPP) (AA 162-312) antibody
ALPP 适用: 人 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal ALP-870 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 小鼠 Monoclonal GM022 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC, ISt 宿主: 兔 Monoclonal ALPP-2899R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-350R unconjugated Recombinant Antibody
anti-Placental Alkaline Phosphatase (ALPP) antibodyALPP 适用: 人 WB, IHC, ELISA 宿主: 小鼠 Monoclonal 3E5 unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (N-Term) antibodyALPP 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 20-143) antibodyALPP 适用: 人 WB, ELISA, IHC (fro), IHC (p), ICC 宿主: 兔 Polyclonal unconjugated
anti-Placental Alkaline Phosphatase (ALPP) (AA 534-792) antibodyALPP 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.25-0.5 μg/mL. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2 cells. Placenta or seminoma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- PLAP (ALPP) (Placental Alkaline Phosphatase (ALPP))
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别名
- PLAP
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背景
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Synonyms: Alkaline phosphatase placental type|Alkaline phosphatase Regan Isozyme|ALP|Alp1|ALPP|Germ-cell alkaline phosphatase|nagao Isozyme|PALP|Placental alkaline phosphatase 1|placental heat-stable alkaline phosphatase|PLAP-1|PLAP1
Gene Symbol: ALPP
Tissue Expression: Placenta
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分子量
- 70 kDa
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基因ID
- 250
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UniProt
- P05187
抗原
-
