pan Keratin 抗体 (acidic) (Biotin)
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Quick Overview for pan Keratin 抗体 (acidic) (Biotin) (ABIN7658453)
抗原
See all pan Keratin (panKRT) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- acidic
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原理
- Cytokeratin, Acidic (Type I or LMW) (Epithelial Marker)(AE-1), Biotin conjugate
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产品特性
- This MAb recognizes the 56. 5 kDa (CK10), 50 kDa (CK14), 50 kDa (CK15), 48 kDa (CK16), 40 kDa (CK19) keratins of the acidic (Type I or LMW) subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e. g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human epidermal keratin
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亚型
- IgG1, kappa
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应用备注
- For coating for ELISA, order Ab without BSA. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: Skin or squamous cell carcinoma (IHC).
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- pan Keratin (panKRT)
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别名
- Keratin
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背景
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Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39
Gene Symbol: KRT77
Tissue Expression: Epidermal cells|Epithelial cells
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分子量
- 40-56.5 kDa
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基因ID
- 374454
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UniProt
- Q7Z794
抗原
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