pan Keratin 抗体
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- 抗原 See all pan Keratin (panKRT) 抗体
- pan Keratin (panKRT)
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适用
- 人, 大鼠, 小鼠, Cow, 猴, 犬, 小鸡, 兔
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This pan Keratin antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human epidermal keratin was used as the immunogen for this pan Cytokeratin antibody.
- 克隆位点
- AE1-AE3
- 亚型
- IgG1
- Top Product
- Discover our top product panKRT Primary Antibody
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anti-pan Keratin (panKRT) antibody
panKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibodypanKRT 适用: 哺乳动物 FACS, WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-11 unconjugated
anti-pan Keratin (panKRT) antibody (FITC)panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 FITC
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 7H8C4 unconjugated
anti-pan Keratin (panKRT) (AA 331-430) antibodypanKRT 适用: 人, 大鼠 IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) (pan) antibodypanKRT 适用: 人, 大鼠, 小鼠 WB, IHC (p), ELISA 宿主: 兔 Polyclonal unconjugated
anti-pan Keratin (panKRT) antibody (PE)panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 PE
anti-pan Keratin (panKRT) antibody (APC)panKRT 适用: 哺乳动物 FACS 宿主: 小鼠 Monoclonal C-11 APC
anti-pan Keratin (panKRT) antibody (Biotin)panKRT 适用: 哺乳动物 FACS, WB, IHC (p), ICC, IP 宿主: 小鼠 Monoclonal C-11 Biotin
anti-pan Keratin (panKRT) antibodypanKRT 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-000R unconjugated Recombinant Antibody
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the pan Cytokeratin antibody AE1 + AE3 to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,for 2 hours at RT,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the pan Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- pan Keratin (panKRT)
- 别名
- Pan Cytokeratin (panKRT 产品)
- 别名
- xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody
- 背景
- Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This pan keratin antibody cocktail recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, which include CK1, CK3-6, CK8, CK10, CK14-16, and CK19. Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The AE1 + AE3 antibody cocktail is a broad spectrum pan cytokeratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. It has been used to characterize the source of various neoplasms and to study the distribution of keratin containing cells in epithelia during normal development and during the development of epithelial neoplasms. It stains cytokeratin present in normal and abnormal human tissues and has shown high sensitivity in the recognition of epithelial cells and carcinomas.
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