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pan Keratin 抗体 (CF®740)

This anti-pan Keratin antibody is a 小鼠 单克隆 antibody detecting pan Keratin in IHC 和 IHC (fp). Suitable for 人, 大鼠, 小鼠, Cow, 猴, Pig, Frog, 豚鼠 和 山羊.
产品编号 ABIN7658424
发货至: 中国

Quick Overview for pan Keratin 抗体 (CF®740) (ABIN7658424)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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人, 大鼠, 小鼠, Cow, 猴, Pig, Frog, 豚鼠, 山羊

宿主

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小鼠

克隆类型

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单克隆

标记

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This pan Keratin antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

KRT-457
  • 原理

    Cytokeratin, multi(KRT/457), CF740 conjugate

    产品特性

    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8), 56.5 kDa (CK10), 53 kDa (CK13) and 45 kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Keratin-enriched preparation from cultured human epithelial cells

    亚型

    IgG1
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL

    说明

    Positive Control: A431 cells, Skin, Colon carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    pan Keratin (panKRT)

    别名

    Keratin, multi

    背景

    Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    Gene Symbol: KRT4

    Tissue Expression: Epidermal cells|Epithelial cells

    分子量

    Multiple

    基因ID

    3851
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