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pan Keratin 抗体 (CF®740)

This anti-pan Keratin antibody is a 小鼠 单克隆 antibody detecting pan Keratin in IF, FACS, WB, IHC 和 IHC (fp). Suitable for 人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊 和 Marmoset.
产品编号 ABIN7658422
发货至: 中国

Quick Overview for pan Keratin 抗体 (CF®740) (ABIN7658422)

抗原

See all pan Keratin (panKRT) 抗体
pan Keratin (panKRT)

适用

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人, 大鼠, 小鼠, Cow, Pig, Frog, 豚鼠, 山羊, Marmoset

宿主

  • 99
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小鼠

克隆类型

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单克隆

标记

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This pan Keratin antibody is conjugated to CF®740

应用范围

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Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

C11
  • 原理

    Cytokeratin, multi(C11), CF740 conjugate

    产品特性

    This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Keratin-enriched preparation from cultured human A431

    亚型

    IgG1
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology formalin-fixed 0.5-1.0 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Immunofluorescence 0.5-1.0 μg/mL. Western blotting 0.5-1.0 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: A431 cells, Skin, Colon carcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    pan Keratin (panKRT)

    别名

    Keratin, multi

    背景

    Synonyms: K1B, KRT1B, Keratin, type II cytoskeletal 1b, K77, CK-1B, Keratin 1B, Keratin-77, Cytokeratin-1B, Type-II Keratin Kb39

    Gene Symbol: KRT4

    Tissue Expression: Epidermal cells|Epithelial cells

    分子量

    Multiple

    基因ID

    3851
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