NGFR 抗体 (CF®740)
Quick Overview for NGFR 抗体 (CF®740) (ABIN7657781)
抗原
See all NGFR 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- NGFR (Nerve Growth Factor Receptor)(NGFR5), CF740 conjugate
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产品特性
- This antibody recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa 1-160 of extracellular domain of NGFR/NTR. NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- NGFR from A875 melanoma cells
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亚型
- IgG1, kappa
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anti-Nerve Growth Factor Receptor (NGFR) (AA 301-400) antibody
NGFR 适用: 人, 大鼠, 小鼠 WB, FACS, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 大鼠 WB, IHC, ELISA 宿主: 小鼠 Monoclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) (AA 281-421) antibodyNGFR 适用: 人 IHC, Diff, StM 宿主: 小鼠 Monoclonal NGFR-1964 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) (AA 188-203), (Extracellular) antibodyKO Validated NGFR 适用: 人 WB, FACS, IHC, IF, IP, IC, LCI 宿主: 兔 Polyclonal unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, Non-Human Primate IHC, Diff, StM 宿主: 兔 Monoclonal NGFR-1997R unconjugated Recombinant Antibody
anti-Nerve Growth Factor Receptor (NGFR) (AA 281-421) antibodyNGFR 适用: 人 IHC, Diff, StM 宿主: 小鼠 Monoclonal rNGFR-1965 unconjugated Recombinant Antibody
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, Non-Human Primate, 兔, Cat, 雪貂 WB, FACS, IHC (p), IP, ICC, ICFC, MS 宿主: 小鼠 Monoclonal NGFR5 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人 FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 2F1C2 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, 小鼠 WB, IHC, IF, ELISA 宿主: 小鼠 Monoclonal 8G3G10 unconjugated
anti-Nerve Growth Factor Receptor (NGFR) antibodyNGFR 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 小鼠 Monoclonal 8G3G10 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Does not react with mouse or rat, others not known. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Melanoma|Neuronal axons, Schwann cells, and perineural cells of peripheral nerves|Soma and axons of sensory neurons, and ganglionic satellite cells|Tumors of nerve sheath differentiation, Schwannoma, Neurofibroma
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- NGFR (Nerve Growth Factor Receptor (NGFR))
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别名
- NGFR
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背景
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Synonyms: CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, Nerve growth factor receptor (NGFR), p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16)
Gene Symbol: NGFR
Tissue Expression: Neural crest cells
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分子量
- 75 kDa
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基因ID
- 4804
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UniProt
- P08138
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途径
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
抗原
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