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NAPSA 抗体 (AA 189-299) (CF®740)

This anti-NAPSA antibody is a 小鼠 单克隆 antibody detecting NAPSA in IHC, WB 和 IHC (fp). Suitable for 人.
产品编号 ABIN7657719
发货至: 中国
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Quick Overview for NAPSA 抗体 (AA 189-299) (CF®740) (ABIN7657719)

抗原

See all NAPSA 抗体
NAPSA (Napsin A Aspartic Peptidase (NAPSA))

适用

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宿主

  • 69
  • 64
小鼠

克隆类型

  • 88
  • 45
单克隆

标记

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This NAPSA antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Western Blotting (WB), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

NAPSA-1238 NAPSA-1239
  • 抗原表位

    • 57
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    AA 189-299

    原理

    Napsin A (Lung Adenocarcinoma Marker)(NAPSA/1238 + NAPSA/1239), CF740 conjugate

    产品特性

    Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B.There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.

    免疫原

    Recombinant human Napsin-A protein fragment (aa189-299)

    亚型

    IgG, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistochemistry (formalin-fixed): 1-2 μg/mL for 30 minutes at RT. Western blot: 1-2 μg/mL for 60 minutes at RT. Flow cytometry: 0.5-1 μg/million cells. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Lung adenocarcinoma

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    NAPSA (Napsin A Aspartic Peptidase (NAPSA))

    别名

    Napsin-A

    背景

    Synonyms: ASP4, Aspartyl protease 4, KAP, Kidney derived aspartic protease like protein (Kdap), NAP1, NAPA, Napsa, napsin A aspartic peptidase, Pronapsin A, SNAPA

    Gene Symbol: NAPSA

    Tissue Expression: Kidney|Lung

    分子量

    37 kDa

    基因ID

    9476

    UniProt

    O96009

    途径

    Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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