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Recombinant MUC2 抗体 (CF®740)

This anti-MUC2 antibody is a 兔 单克隆 antibody detecting MUC2 in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7657371
发货至: 中国

Quick Overview for Recombinant MUC2 抗体 (CF®740) (ABIN7657371)

抗原

See all MUC2 抗体
MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This MUC2 antibody is conjugated to CF®740

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

MLP-2970R
  • 原理

    MUC2 (Mucin 2) (MLP/2970R), CF740 conjugate

    产品特性

    This antibody recognizes a single glycoprotein of 520 kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Its epitope has been defined as GTQTP (GlyThrGlnThrPro). Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon, in about 65 % of colonic carcinomas and about 40 % of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant full-length human MUC2 protein

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris-HCl buffer pH 10.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: LS174T cells or small intestine.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    MUC2 (Mucin 2, Oligomeric Mucus/gel-Forming (MUC2))

    别名

    MUC2

    背景

    Synonyms: Mucin-2, Intestinal mucin-2, Mucin like protein (MLP), SMUC

    Gene Symbol: MUC2

    Tissue Expression: Goblet cells|Intestine

    分子量

    520 kDa

    基因ID

    4583

    UniProt

    Q02817
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