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Recombinant MLANA 抗体 (CF®740)

This CF®740-conjugated 兔 单克隆 anti-MLANA antibody (Clone MLANA-1409R) specifically detects MLANA in WB, IHC 和 IHC (fp). The antibody is reactive with 人, 小鼠 和 大鼠 samples.
产品编号 ABIN7656741
发货至: 中国
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中国
北京 101111
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Room 801-803
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Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant MLANA 抗体 (CF®740) (ABIN7656741)

抗原

See all MLANA 抗体
MLANA (Melan A (MLANA))

抗体类型

Recombinant Antibody

适用

  • 214
  • 115
  • 90
  • 36
  • 16
  • 1
人, 小鼠, 大鼠

宿主

  • 109
  • 104
  • 1
  • 1

克隆类型

  • 140
  • 75
单克隆

标记

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  • 2
  • 1
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This MLANA antibody is conjugated to CF®740

应用范围

  • 147
  • 111
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  • 84
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  • 53
  • 26
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  • 23
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  • 15
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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

MLANA-1409R
  • 原理

    MART-1 / Melan-A / MLANA (Melanoma Marker) (MLANA/1409R), CF740 conjugate

    产品特性

    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human full-length MLANA protein

    亚型

    IgG, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    MLANA (Melan A (MLANA))

    别名

    MART-1

    背景

    Synonyms: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

    Gene Symbol: MLANA

    Tissue Expression: Melanocytes

    分子量

    20-22 kDa (doublet)

    基因ID

    2315

    UniProt

    Q16655
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