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MLANA 抗体

MLANA 适用: 人, 小鼠, 大鼠 WB, IF, FACS, IHC, ISt, StM 宿主: 小鼠 Monoclonal M2-9E3 unconjugated
产品编号 ABIN6939392
发货至: 中国
  • 抗原 See all MLANA 抗体
    MLANA (Melan A (MLANA))
    适用
    • 185
    • 102
    • 84
    • 33
    • 15
    人, 小鼠, 大鼠
    宿主
    • 97
    • 87
    • 1
    小鼠
    克隆类型
    • 114
    • 71
    单克隆
    标记
    • 75
    • 15
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    • 8
    • 8
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    • 4
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    • 2
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    • 1
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    This MLANA antibody is un-conjugated
    应用范围
    • 133
    • 101
    • 100
    • 77
    • 58
    • 53
    • 28
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    • 26
    • 22
    • 13
    • 11
    • 8
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    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunostaining (ISt), Staining Methods (StM)
    特异性
    This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.
    纯化方法
    Purified by Protein A/G
    免疫原
    Recombinant hMART-1 protein
    克隆位点
    M2-9E3
    亚型
    IgG2b kappa
    Top Product
    Discover our top product MLANA Primary Antibody
  • 应用备注

    Positive Control: SK-MEL-13 and SK-MEL-19 melanoma cell lines. Human melanoma tissues.

    Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

    限制
    仅限研究用
  • 浓度
    200 μg/mL
    缓冲液
    10 mM PBS with 0.05 % BSA & 0.05 % azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-80 °C
    储存方法
    Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
    有效期
    24 months
  • 抗原
    MLANA (Melan A (MLANA))
    别名
    MLANA (MLANA 产品)
    别名
    A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
    分子量
    20-22kDa (doublet)
    基因ID
    2315
    UniProt
    Q16655
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