LIG1 抗体
Quick Overview for LIG1 抗体 (ABIN7656378)
抗原
See all LIG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- DNA Ligase 1(1A9)
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产品特性
- DNA Ligase I maintains the major DNA Ligase activity in proliferating cells by joining Okazaki fragments during lagging strand DNA replication. Human DNA Ligase I also has an essential role in DNA repair pathways, where it catalyzes the formation of phosphodiester bonds between adjacent 5' phosphoryl and 3' hydroxy termini at single breaks in duplex DNA molecules. In addition, DNA Ligase I plays a role in sealing nicks during excision repair. Similar to other DNA ligases, DNA Ligase I is built around a common catalytic core. Increased levels of DNA Ligase I are found in human tumors, as compared to benign tissues, as well as in peripheral blood lymphocytes. DNA Ligase I activity is altered in the chromosomal breakage deficit Bloom's syndrome (BS). Individuals with BS either have decreased levels of abnormally thermolabile DNA Ligase I or possess a dimeric form of this enzyme. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Full-length native cow LIG1 protein
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亚型
- IgG1, kappa
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anti-Ligase I, DNA, ATP-Dependent (LIG1) antibody
LIG1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-919) antibodyLIG1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal AG12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 565-790) antibodyLIG1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人 WB, ELISA, IHC 宿主: 兔 Monoclonal 7B2 unconjugated Recombinant Antibody
anti-Ligase I, DNA, ATP-Dependent (LIG1) (Internal Region) antibodyLIG1 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 1-919) antibodyLIG1 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 大鼠, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (AA 810-920) antibodyLIG1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 10G12 unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) antibodyLIG1 适用: 人, 小鼠 IF 宿主: 兔 Polyclonal unconjugated
anti-Ligase I, DNA, ATP-Dependent (LIG1) (C-Term) antibodyLIG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Western blot 0.5-1 μg/mL, ELISA, Immunoprecipitation, Optimal dilution for a specific application should be determined.
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说明
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Positive Control: MOLT-4, PC-3 or Jurkat cell lysates.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 mg/mL
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缓冲液
- PBS, 0.05 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- LIG1 (Ligase I, DNA, ATP-Dependent (LIG1))
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别名
- DNA Ligase 1
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背景
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Synonyms: DNA ligase 1, DNA ligase I, DNLI1_HUMAN, LIG 1, lig1, Ligase I DNA ATP dependent, MGC117397, MGC130025, Polydeoxyribonucleotide synthase [ATP] 1
Gene Symbol: LIG1
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分子量
- 133 kDa
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基因ID
- 3978
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UniProt
- P18858
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途径
- Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA
抗原
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