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Recombinant LGALS1/Galectin 1 抗体 (AA 12-108) (CF®740)

This 兔 单克隆 antibody specifically detects LGALS1/Galectin 1 in WB, IHC 和 IHC (fp). It exhibits reactivity toward 人.
产品编号 ABIN7656319
发货至: 中国
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Quick Overview for Recombinant LGALS1/Galectin 1 抗体 (AA 12-108) (CF®740) (ABIN7656319)

抗原

See all LGALS1/Galectin 1 (LGALS1) 抗体
LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

抗体类型

Recombinant Antibody

适用

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宿主

  • 156
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克隆类型

  • 113
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  • 2
单克隆

标记

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This LGALS1/Galectin 1 antibody is conjugated to CF®740

应用范围

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Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

GAL1-2499R
  • 抗原表位

    • 35
    • 27
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    • 2
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    • 1
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    • 1
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    • 1
    AA 12-108

    原理

    Galectin-1 / Human Placental Lactogen (hPL) (GAL1/2499R), CF740 conjugate

    产品特性

    Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant human Galectin-1 protein fragment (aa12-108)

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. ELISA: 2-4 μg/m for coating order Ab without BSA. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % rBSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

    别名

    Galectin-1

    背景

    Synonyms: 14 kDa laminin-binding protein, Beta-galactoside-binding lectin L-14-I, Gal-1, GAL1, Galaptin, Galectin-1, HLBP14, HPL, Lactose-binding lectin 1, Lect14, Lectin galactoside-binding soluble 1, LGALS1, MAPK activating protein MP12, S-Lac lectin 1

    Gene Symbol: LGALS1

    分子量

    14 kDa

    基因ID

    3956

    UniProt

    P09382

    途径

    Carbohydrate Homeostasis
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