Keratin 7/17 抗体 (CF®594)
Quick Overview for Keratin 7/17 抗体 (CF®594) (ABIN7656245)
抗原
See all Keratin 7/17 (KRT7/17) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin 7/17(C-46), CF594 conjugate
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产品特性
- Recognizes intermediate filament proteins (IFP's) of 55 kDa and 46 kDa, which are identified as cytokeratin 7 and Cytokeratin 17 respectively. On formalin-fixed, paraffin embedded sections, C-46 reacts with keratin 7 only. Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. Cytokeratins have been found to be useful markers of tissue differentiation, which is directly applicable to the characterization of malignant tumors. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Cytokeratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. Cytokeratin 17 is expressed in epithelial cells of various origins, such as bronchial epithelial cells and skin appendages. Cytokeratin 17 antibody marks basal cell differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Cytoskeletal preparation from HeLa cells (human cervical carcinoma cells)
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亚型
- IgG1, kappa
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anti-Keratin 7/17 (KRT7/17) antibody
KRT7/17 适用: 人, Cow, Pig WB, IP, ICC, IHC (p) 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow IHC, WB, FACS, IF, StM 宿主: 小鼠 Monoclonal C-46 unconjugated
anti-Keratin 7/17 (KRT7/17) antibodyKRT7/17 适用: 人, Cow, Pig IHC, WB, IP, IF/ICC 宿主: 小鼠 Monoclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: HeLa or ME-180 cells. Skin or hepatocellular carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Keratin 7/17 (KRT7/17)
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别名
- Keratin 7/17
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背景
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Synonyms: CK-7; K2C7; Keratin 55K Type II Cytoskeletal; Keratin Simple Epithelial Type 1 K7; Keratin Type II Cytoskeletal 7; Krt2-7; KRT7; Sarcolectin; SCL; Type II Mesothelial Keratin K7; Type-II Keratin Kb7; CK17; K17; Keratin Type I Cytoskeletal 17; Keratin-17; KRT17; PCHC1
Gene Symbol: KRT7 & KRT17
Tissue Expression: Epithelial cells
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分子量
- 55 kDa (CK7), 46 kDa (CK17)
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基因ID
- 3855, 3872
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UniProt
- P08729, Q04695
抗原
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