KRT15 抗体 (CF®740)
Quick Overview for KRT15 抗体 (CF®740) (ABIN7655949)
抗原
See all KRT15 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin 15 (KRT15) (Basal Cell Marker) (KRT15/1699), CF740 conjugate
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产品特性
- Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Keratin 15 is a type I keratin which is expressed only in basal keratinocytes in stratified epithelia and does not appear to have a natural type II expression partner. Keratin 15 is down regulated in activated keratinocytes. Cytokeratin 15 is a specific marker of stem cells of the hair-follicle bulge and may be a useful marker for diagnosis between basal cell carcinoma (BCC) and trichoepithelioma. Trichoblastoma are benign neoplasms of follicular differentiation frequently found in nevus sebaceous. Many morphologic features are shared with nodular basal cell carcinoma, sometimes rendering a diagnosis difficult. Trichoblastoma and BCC show variable expression of Cytokeratin 15 and Cytokeratin 19, and absence of hair keratins.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human KRT15 protein
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亚型
- IgG2a, kappa
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anti-Keratin 15 (KRT15) antibody
KRT15 适用: 人 IHC, FACS, StM 宿主: 小鼠 Monoclonal KRT15-2554 unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal KRT15-2957 unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人 WB, IHC, FACS, StM 宿主: 小鼠 Monoclonal KRT15-2958 unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人 IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 6E7 unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人, 大鼠, Cow IHC, FACS, StM 宿主: 兔 Monoclonal KRT15-2103R unconjugated Recombinant Antibody
anti-Keratin 15 (KRT15) (C-Term) antibodyKRT15 适用: 人, 小鼠, Cow WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 15 (KRT15) (AA 1-456) antibodyKRT15 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Keratin 15 (KRT15) (AA 1-456) antibodyKRT15 适用: 人 WB, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Keratin 15 (KRT15) antibodyKRT15 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-615M unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (frozen or formalin) 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: A431 cells. Skin or Colon
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- KRT15 (Keratin 15 (KRT15))
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别名
- Keratin 15
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背景
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Synonyms: CK15, Cytokeratin 15, K1CO, Ka15, Keratin 15 basic, Keratin 15 beta, Keratin complex 1 acidic gene 15, Keratin type I cytoskeletal 15, KRT15, KRTB, KRTL15, Type I cytoskeletal 15, Type I keratin Ka15
Gene Symbol: KRT15
Tissue Expression: Epidermal cells
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分子量
- 52 kDa
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基因ID
- 3866
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UniProt
- P19012
抗原
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