Recombinant Keratin 10 抗体 (CF®594)
Quick Overview for Recombinant Keratin 10 抗体 (CF®594) (ABIN7655888)
抗原
See all Keratin 10 (KRT10) 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) (rKRT10/844), CF594 conjugate
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产品特性
- This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human KRT10 protein.
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亚型
- IgG1, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: A431, HeLa, MCF7 cells. Esophagus or tonsil.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Keratin 10 (KRT10)
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别名
- Keratin 10
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背景
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Synonyms: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
Gene Symbol: KRT10
Tissue Expression: Epidermal cells
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分子量
- 56.5 kDa
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基因ID
- 3858
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UniProt
- P13645
抗原
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