Recombinant Histone H1 抗体
Quick Overview for Recombinant Histone H1 抗体 (ABIN7655107)
抗原
See all Histone H1 抗体抗体类型
适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Histone H1 (Pan Nuclear Marker)(rAE-4)
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产品特性
- Please note that this antibody is a recombinant Mouse version of original anti-histone H1 antibody (Clone AE-4). Because the variable heavy (VH) and variable light (VL) domains are the same, recombinant antibody has the same exact reactivity as the original AE-4 MAb. There are several advantages of producing a recombinant version of a monoclonal antibody. For example, a recombinant antibody is a purer preparation of active immunoglobulin with no contaminating non-functional intact Ig or free light/heavy chains. Secondly, antibody can always be produced, even if hybridoma line is lost. Moreover, it adds the flexibility of converting the antibody to any species, isotype or format. Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. This antibody is extensively used as a pan-nuclear marker. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant full-length human Histone H1 protein
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亚型
- IgG2a, kappa
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anti-Histone H1 antibody
适用: 人, 小鼠, 大鼠 IF, IHC, FACS, StM 宿主: 小鼠 Monoclonal 1415-1 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, IHC, FACS, StM, CUT&RUN 宿主: 小鼠 Monoclonal AE-4 unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, IHC, FACS, StM 宿主: 小鼠 Monoclonal r1415-1 unconjugated Recombinant Antibody
anti-Histone H1 (AA 1-105) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, IHC, FACS, StM 宿主: 兔 Monoclonal HH1-1784R unconjugated Recombinant Antibody
anti-Histone H1 (acLys52) antibody适用: 人, 小鼠, 大鼠 FACS, IHC (p), ELISA, IF (cc), IF (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 antibody适用: 人, 小鼠, 大鼠 IF, IHC, FACS, StM 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 150-207) antibody适用: 人 IHC, WB 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (Lys1535) antibody适用: 人, 小鼠 IF, IHC, WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Histone H1 (AA 1-194) antibody适用: 人 IF, IHC, WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS, no BSA, no azide
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Store at -20 °C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- Histone H1
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背景
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Synonyms: H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1F0, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Oocyte-specific histone H1, Testicular H1 histone
Gene Symbol: H1
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分子量
- ~30 kDa
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基因ID
- 3005
抗原
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