GLG1 抗体 (CF®740)
Quick Overview for GLG1 抗体 (CF®740) (ABIN7654632)
抗原
See all GLG1 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GLG1 (Golgi Complex)(GLG1/970), CF740 conjugate
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产品特性
- This MAb recognizes a protein of 134 kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Golgi fraction from human liver cells
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亚型
- IgG1, kappa
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anti-Golgi Glycoprotein 1 (GLG1) (AA 1048-1145) antibody
GLG1 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Golgi Glycoprotein 1 (GLG1) (AA 1152-1179), (C-Term) antibodyGLG1 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal RB24297 unconjugated
anti-Golgi Glycoprotein 1 (GLG1) (C-Term) antibodyGLG1 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Golgi Glycoprotein 1 (GLG1) antibodyGLG1 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal GLG1-970 unconjugated
anti-Golgi Glycoprotein 1 (GLG1) (AA 974-1203) antibodyGLG1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Does not react with mouse or rat, others not known. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Immunocytochemistry Acetone or paraformaldehyde fixed 0.5-1 μg/mL. Western blotting 0.5-1.0 μg/mL. Flow Cytometry 0.5-1.0 μg/million cells in 0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- GLG1 (Golgi Glycoprotein 1 (GLG1))
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别名
- GLG1
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背景
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Synonyms: CFR-1, Cysteine-rich fibroblast growth factor receptor, E-selectin ligand-1 (ESL-1), Golgi Glycoprotein 1 (GLG1), Golgi apparatus protein 1, Golgi sialoglycoprotein MG-160, Slectin, endothelial cell, ligand
Gene Symbol: GLG1
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分子量
- 134 kDa
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基因ID
- 2734
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UniProt
- Q92896
抗原
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