FAT Atypical Cadherin 2 (FAT2) 抗体 (CF®740)
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北京 101111
Quick Overview for FAT Atypical Cadherin 2 (FAT2) 抗体 (CF®740) (ABIN7653924)
抗原
See all FAT Atypical Cadherin 2 (FAT2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- Protocadherin FAT2 (FAT Atypical Cadherin 2) (8C5), CF740 conjugate
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产品特性
- This antibody recognizes a protein of 480 kDa, which is identified as FAT2. The cadherins represent a family of Ca2 -dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including beta-catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Purified recombinant human FAT2 fusion protein
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亚型
- IgG1, kappa
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anti-FAT Atypical Cadherin 2 (FAT2) antibody
FAT2 适用: 人 FACS, IF, IHC, StM 宿主: 小鼠 Monoclonal 8C5 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Esophagus, Cervix, Uterus, Tonsil or Cerebellum.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- FAT Atypical Cadherin 2 (FAT2)
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别名
- Protocadherin-FAT2
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背景
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Synonyms: FAT tumor suppressor homolog 2 (HFAT2), cadherin family member 8 (CDHF8), cadherin-related family member 9 (CDHR9), multiple EGF-like domains protein 1, multiple epidermal growth factor-like domains 1 (MEGF1), multiple epidermal growth factor-like domains protein 1, protocadherin FAT2
Gene Symbol: FAT2
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分子量
- 480 kDa
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基因ID
- 2196
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UniProt
- Q9NYQ8
抗原
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