FBXO5 抗体 (AA 1-250) (CF®740)
Quick Overview for FBXO5 抗体 (AA 1-250) (CF®740) (ABIN7653910)
抗原
See all FBXO5 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 1-250
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原理
- EMI1(EMI1/1176), CF740 conjugate
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产品特性
- This antibody recognizes a 56 kDa protein, which is identified as Early Mitotic Inhibitor-1 (EMI1). It regulates mitosis by inhibiting the anaphase promoting complex/cyclosome (APC). Emi1 is a conserved F box protein containing a zinc-binding region essential for APC inhibition. The Emi1 protein functions to promote cyclin A accumulation and S phase entry in somatic cells by inhibiting the APC complex. At the G1-S transition, Emi1 is transcriptionally induced by the E2F transcription factor. Emi1 overexpression accelerates S-phase entry and can override a G1 block caused by overexpression of Cdh1 or the E2F-inhibitor p105 retinoblastoma protein (pRb). Depleting cells of Emi1 through RNA interference prevents accumulation of cyclin A and inhibits S phase entry.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment (203 amino acid residues between aa 1-250) of human EMI1 protein
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亚型
- IgG2a, kappa
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anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibody
FBXO5 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人, 犬, Pig, 兔 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 358-446) antibodyFBXO5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5H7 unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 326-375) antibodyFBXO5 适用: 人, 犬, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (C-Term) antibodyFBXO5 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-F-Box Protein 5 (FBXO5) (AA 1-250) antibodyFBXO5 适用: 人 WB, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal EMI1-1176 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Western blotting 1-2 μg/mL. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HeLa, 293 or HepG2 cells. Ovarian carcinoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- FBXO5 (F-Box Protein 5 (FBXO5))
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别名
- EMI1
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背景
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Synonyms: Early Mitotic Inhibitor-1 (EMI1), FBX5, Fbxo31
Gene Symbol: FBXO5
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分子量
- 56 kDa
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基因ID
- 26271
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UniProt
- Q9UKT4
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途径
- Mitotic G1-G1/S Phases
抗原
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