EPO 抗体 (AA 28-162) (CF®740)
Quick Overview for EPO 抗体 (AA 28-162) (CF®740) (ABIN7653729)
抗原
See all EPO 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 28-162
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原理
- Erythropoietin (EPO) (EPO/1368), CF740 conjugate
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产品特性
- This antibody recognizes a protein of about 37 kDa, which is identified as Erythropoietin (EPO). Erythropoietin is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment of human EPO protein (aa28-162)
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亚型
- IgG
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anti-Erythropoietin (EPO) (AA 28-193) antibody
EPO 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 101-193) antibodyEPO 适用: 人, 大鼠 WB, ELISA, FACS, IHC (p), IF (p), IF (cc), IHC (fro), DB 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-193) antibodyEPO 适用: 人 WB, IHC 宿主: 小鼠 Monoclonal C3 unconjugated
anti-Erythropoietin (EPO) antibodyEPO 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-162) antibodyEPO 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1368 unconjugated
anti-Erythropoietin (EPO) (AA 28-162) antibodyEPO 适用: 人 IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal EPO-1367 unconjugated
anti-Erythropoietin (EPO) (AA 20-48) antibodyEPO 适用: 人, 小鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-191) antibodyEPO 适用: 人 IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) antibodyEPO 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Erythropoietin (EPO) (AA 28-193) antibodyEPO 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4G7 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin) 4-8 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HepG2 Cells. Heart or Kidney.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- EPO (Erythropoietin (EPO))
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别名
- Erythropoietin
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物质类
- Hormone
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背景
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Synonyms: EP, EPO alpha, EPO, Epoetin, Erythropoietin, MVCD2
Gene Symbol: EPO
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分子量
- 37 kDa
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基因ID
- 2056
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UniProt
- P01588
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途径
- JAK/STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity
抗原
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