ENO2/NSE 抗体 (AA 416-433) (CF®405S)
Quick Overview for ENO2/NSE 抗体 (AA 416-433) (CF®405S) (ABIN7653500)
抗原
See all ENO2/NSE (ENO2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 416-433
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原理
- NSE gamma (Neuron Specific Enolase, gamma) (Neuroendocrine Marker)(NSE-P2), CF405S conjugate
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产品特性
- This monoclonal antibody recognizes a protein of about 50 kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas. It it be usually employed in combination with other markers such as Synaptophysin, Chromogranin A, and Neurofilament. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- A synthetic peptide of human NSE gamma (around aa416-433)
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亚型
- IgG1, kappa
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应用备注
- Optimal working dilution should be determined by the investigator.
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说明
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Positive Control: HepG2, SH-SY-5Y, HeLa or Y79 cells. Pancreas, Cerebellum or Pheochromocytoma.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- ENO2/NSE (ENO2) (Enolase 2 (Gamma, Neuronal) (ENO2))
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别名
- NSE-Gamma
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背景
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Synonyms: 2-phospho-D-glycerate hydrolyase, ENO2, ENOG, Enolase 2 gamma neuronal, Enolase2, Gamma-enolase, Neural enolase, Neuron specific gamma enolase, Neuron-specific enolase, NSE
Gene Symbol: ENO2
Tissue Expression: Neurons
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分子量
- ~50 kDa
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基因ID
- 2026
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UniProt
- P09104
抗原
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