CPK 抗体 (Phosphospecific) (CF®740)
This anti-CPK antibody is a 小鼠 单克隆 antibody detecting CPK in . Suitable for 人.
Quick Overview for CPK 抗体 (Phosphospecific) (CF®740) (ABIN7652699)
抗原
See all CPK 抗体
CPK
(Creatine Phosphokinase (CPK))
适用
人
宿主
小鼠
克隆类型
单克隆
标记
This CPK antibody is conjugated to CF®740
应用范围
请咨询
克隆位点
2ba6
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抗原表位
- Phosphospecific
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原理
- Creatine Phosphokinase(2ba6), CF740 conjugate
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产品特性
- Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments. They provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB. It shows minimal reactivity with other human serum proteinsPrimary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Human CKBB protein
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亚型
- IgG1, kappa
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Not suitable for FFPE sections. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: Cerebellum
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CPK (Creatine Phosphokinase (CPK))
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别名
- Creatine Phosphokinase
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背景
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Synonyms: B-CK, BB-CK, creatine kinase-B, Creatine kinase B chain, CKBB creatine kinase B-type, creatine kinase, brain, CKBB, Brain creatine kinase, Creatine kinase B-type, Creatine Kinase BB Isoenzyme, Creatine kinase brain, Creatine phosphokinase BB
Gene Symbol: CKB
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分子量
- 43 kDa (Monomer), 86 kDa (Dimer)
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基因ID
- 1152
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UniProt
- P12277
抗原
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