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CPK 抗体

CPK 适用: 兔 IF, WB, ELISA 宿主: 绵羊 Polyclonal unconjugated
产品编号 ABIN459287
发货至: 中国
  • 抗原 See all CPK 抗体
    CPK (Creatine Phosphokinase (CPK))
    适用
    • 9
    • 5
    宿主
    • 9
    • 5
    绵羊
    克隆类型
    • 9
    • 5
    多克隆
    标记
    • 4
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CPK antibody is un-conjugated
    应用范围
    • 14
    • 9
    • 9
    • 5
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    Immunofluorescence (IF), Western Blotting (WB), ELISA
    特异性
    Cross-reactivities against enzymes of other sources may occur but have not been determined.
    产品特性
    IgG fraction of polyclonal sheep antiserum to creatine phosphokinase from rabbit muscle
    纯化方法
    The IgG (7S) fraction is prepared from the antiserum by ammonium sulphate precipitation and ion exchange chromatography.
    免疫原
    Creatine phosphokinase isolated and purified from rabbit muscle. Freund’s complete adjuvant is used in the first step of the immunization procedure.
    亚型
    IgG
  • 应用备注
    This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer’s own choice.
    限制
    仅限研究用
  • 状态
    Lyophilized
    浓度
    IgG protein concentration 10 mg/ml. No foreign proteins added.
    缓冲液
    Purified hyperimmune sheep IgG lyophilised from a solution in phosphate buffered saline (PBS, pH 7.2).
    储存液
    Without preservative
    储存条件
    4 °C/-20 °C
    储存方法
    The lyophilised IgG fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. It is reconsti tuted by adding 1.0 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly at a mbient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
  • 抗原
    CPK (Creatine Phosphokinase (CPK))
    别名
    Creatine Phosphokinase (CPK 产品)
    背景
    The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
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