Clathrin Heavy Chain (CLTC) (N-Term) 抗体 (CF®740)
Quick Overview for Clathrin Heavy Chain (CLTC) (N-Term) 抗体 (CF®740) (ABIN7652447)
抗原
See all Clathrin Heavy Chain (CLTC) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
 - N-Term
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原理
 - Clathrin, Heavy Chain (CLTC/1431), CF740 conjugate
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产品特性
 - This antibody recognizes protein of 192 kDa, which is identified as Clathrin Heavy Chain. Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin heavy chain (HC) is composed of a terminal globular domain, a distal segment and a proximal segment containing a light chain-binding site. The proximal segment of the Clathrin HC protein is essential for interactions between Clathrin heavy chains and light chains, which result in the formation of the triskelion structure.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
 - Purified recombinant N-terminal fragment of human Clathrin Heavy Chain
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亚型
 - IgG1, kappa
 
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应用备注
 - For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: HeLa cells. Placenta or Prostate Carcinoma.
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限制
 - 仅限研究用
 
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状态
 - Liquid
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浓度
 - 0.1 mg/mL
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缓冲液
 - PBS, 0.1 % rBSA, 0.05 % azide
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储存液
 - Sodium azide
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注意事项
 - This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
 - Protect from light
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储存条件
 - 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
 - 24 months
 
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