CD80 抗体 (AA 35-142) (CF®568)
Quick Overview for CD80 抗体 (AA 35-142) (CF®568) (ABIN7652050)
抗原
See all CD80 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- AA 35-142
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原理
- CD80 (B7-1)(C80/2776), CF568 conjugate
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产品特性
- T cell proliferation and lymphokine production are triggered by occupation of the TCR by antigen, followed by a costimulatory signal that is delivered by a ligand expressed on antigen presenting cells. The B7-related cell surface proteins CD80 (B7-1) and CD86 (B7-2) are expressed on antigen presenting cells bind the homologous T cell receptors CTLA-4 (cytotoxic T lymphocyte-associated protein-4) and CD28 and trigger costimulatory signals for optimal T cell activation. CTLA-4 shares 31 % overall amino acid identity with CD28 and it has been proposed that CD28 and CTLA-4 are functionally redundant. SLAM is a novel receptor on T cells that, when engaged, potentiates T cell expansion in a CD28-independent manner. B7, also designated BB1, is another ligand or counter receptor for CD28 and CTLA-4 that is expressed on the antigen-presenting cell. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant fragment of human CD80 protein (around aa 35-142)
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亚型
- IgG2b, kappa
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anti-CD80 (CD80) (AA 35-288) antibody
CD80 适用: 人 FACS, ELISA, IHC 宿主: 小鼠 Monoclonal 1G3D7 unconjugated
anti-CD80 (CD80) (AA 251-321) antibodyCD80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD80 (CD80) antibodyCD80 适用: 小鼠, 犬 FACS, IP, IHC (fro) 宿主: 仓鼠 Monoclonal 16-10A1 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibodyCD80 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-233 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibody (FITC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 FITC
anti-CD80 (CD80) (Extracellular Domain) antibody (PerCP)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PerCP
anti-CD80 (CD80) (Extracellular Domain) antibody (PE)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PE
anti-CD80 (CD80) (Extracellular Domain) antibody (APC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 APC
anti-CD80 (CD80) antibodyCD80 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2A2 unconjugated
anti-CD80 (CD80) (AA 111-265) antibodyCD80 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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应用备注
- For coating for ELISA, order Ab without BSA. Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Optimal dilution and staining procedure for a specific application should be determined by user. Recommended starting concentrations for titration are 1-2 μg/mL for most applications, or 1 μg/million cells/100 μLfor flow cytometry
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说明
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Positive Control: Raji or Ramos Cells. Tonsil, Spleen or Thymus
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % BSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CD80
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别名
- CD80
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背景
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Synonyms: Activation B7-1 antigen, B lymphocyte activation antigen B7, B7, B7-1, BB1, CD28 antigen ligand 1, CD28LG, CD28LG1, CD80, Costimulatory factor CD80, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
Gene Symbol: CD80
Tissue Expression: Antigen presenting cells
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分子量
- 60 kDa
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基因ID
- 941
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UniProt
- P33681
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途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
抗原
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