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CD68 抗体 (AA 150-221) (CF®647)

This anti-CD68 antibody is a 小鼠 单克隆 antibody detecting CD68 in IHC 和 IHC (fp). Suitable for 人.
产品编号 ABIN7651875
发货至: 中国

Quick Overview for CD68 抗体 (AA 150-221) (CF®647) (ABIN7651875)

抗原

See all CD68 抗体
CD68 (CD68 Molecule (CD68))

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This CD68 antibody is conjugated to CF®647

应用范围

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Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

C68-2501
  • 抗原表位

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    AA 150-221

    原理

    CD68 (Macrophage Marker) (C68/2501), CF647 conjugate

    产品特性

    This antibody recognizes a glycoprotein of 110 kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Recombinant fragment of human CD68 protein (around aa 150-221)

    亚型

    IgG2c, kappa
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Flow cytometry: 0.5-1 μg/million cells. Immunofluorescence: 0.5-1 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes. Western: 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: Tonsil, lymph node, or spleen

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Protect from light

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Protect from light

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    CD68 (CD68 Molecule (CD68))

    别名

    CD68

    背景

    Synonyms: GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1

    Gene Symbol: CD68

    Tissue Expression: Monocytes/macrophages

    分子量

    ~110 kDa

    基因ID

    968

    UniProt

    P34810
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