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Recombinant CD6 抗体 (Biotin)

This Biotin-conjugated 兔 单克隆 anti-CD6 antibody (Clone C6-2884R) specifically detects CD6 in FACS, IF, IHC 和 IHC (fp). The antibody is reactive with 人 samples.
产品编号 ABIN7651737
发货至: 中国
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中国
北京 101111
No. 88 KeChuang 6th Street
Beijing Economic Technological Development Area
Room 801-803
4A Biotech Co.,Ltd.
Tel +86 (0512) 65829739 传真 +86 (010) 6788 5057

Quick Overview for Recombinant CD6 抗体 (Biotin) (ABIN7651737)

抗原

See all CD6 抗体
CD6

抗体类型

Recombinant Antibody

适用

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宿主

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克隆类型

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单克隆

标记

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This CD6 antibody is conjugated to Biotin

应用范围

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Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))

克隆位点

C6-2884R
  • 原理

    CD6 (Negative Marker of T-regulatory Cells) (C6/2884R), Biotin conjugate

    产品特性

    CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

    免疫原

    Human recombinant full-length CD6 protein

    亚型

    IgG
  • 应用备注

    Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunohistology (formalin): 0.5-1 μg/mL for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined by user

    说明

    Positive Control: CCRF-CEM, Jurkat cells, Tonsil

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 mg/mL

    缓冲液

    PBS, 0.1 % BSA, 0.05 % azide

    储存液

    Sodium azide

    注意事项

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    4 °C

    储存方法

    Stable at room temperature or 37°C for 7 days.

    Store at 2 to 8°C. Protect fluorescent conjugates from light

    有效期

    24 months
  • 抗原

    CD6

    别名

    CD6

    背景

    Synonyms: T12, TP120

    Gene Symbol: CD6

    Tissue Expression: B-cells|T-cells

    分子量

    90-130 kDa

    基因ID

    923

    UniProt

    P30203
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