CD44 Standard 抗体 (CF®740)
Quick Overview for CD44 Standard 抗体 (CF®740) (ABIN7651493)
抗原
See all CD44 Standard (CD44s) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- CD44 Standard(HCAM/1097), CF740 conjugate
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产品特性
- Recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human HCAM protein
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亚型
- IgG1, kappa
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anti-CD44 Standard (CD44s) antibody
CD44s 适用: 人, Non-Human Primate FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal HCAM-918 unconjugated
anti-CD44 Standard (CD44s) antibodyCD44s 适用: 人, Baboon, Green Monkey FACS, IF, IHC (f), WB 宿主: 小鼠 Monoclonal 156-3C11 unconjugated
anti-CD44 Standard (CD44s) antibodyCD44s 适用: 人, 小鼠 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal DF1485 unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 0.5-1 μg/mL. Immunohistology formalin-fixed 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HeLa cells or paracortex in tonsil or lymph node.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- CD44 Standard (CD44s)
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别名
- CD44 Standard
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背景
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Synonyms: LHR, BA-1, chondroitin sulfate proteoglycan 8 (CSPG8), Epican, Extracellular Matrix Receptor III (ECM III), GP90 Lymphocyte Homing Adhesion Receptor, HCAM, HCELL, hematopoietic cell E- and L-selectin ligand, Heparan Sulfate Proteoglycan, Hermes Antigen, HAS, HUTCH I, Hyaluronate Receptor, Indian blood group, Inlu Related p80 Glycoprotein, Ly 24, MDU2, MDU3, MIC4, MUTCH I, Phagocytic Glycoprotein 1 (PGP-1)
Gene Symbol: CD44
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分子量
- 80-95 kDa
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基因ID
- 960
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UniProt
- P16070
抗原
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