CD44 Standard 抗体
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- 抗原 See all CD44 Standard (CD44s) 抗体
- CD44 Standard (CD44s)
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适用
- 人, Non-Human Primate
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CD44 Standard antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- 原理
- Mouse Monoclonal anti-CD44 Standard (HCAM/918), Purified, BSA-free
- 产品特性
- This antibody recognizes a cell surface glycoprotein of 80-95 kDa (CD44) on lymphocytes, monocytes, and granulocytes (Leucocyte Typing Workshop V). Its epitope is resistant to digestion by trypsin and chymotrypsin. The CD44 family of glycoproteins exists in a number of variant isoforms, the most common being the standard 85-95 kDa or hematopoietic variant (CD44s). Higher molecular weight isoforms are described in epithelial cells (CD44v), which are believed to function in intercellular adhesion and stromal binding. CD44 immunostaining is commonly used for the discrimination of urothelial transitional cell carcinoma in-situ from non-neoplastic changes in the urothelium. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- 纯化方法
- Purified
- 免疫原
- Recombinant human HCAM protein
- 克隆位点
- HCAM-918
- 亚型
- IgG2a kappa
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anti-CD44 Standard (CD44s) antibody
CD44s 适用: 人, Baboon, Green Monkey FACS, IF, IHC (f), WB 宿主: 小鼠 Monoclonal 156-3C11 unconjugated
anti-CD44 Standard (CD44s) antibodyCD44s 适用: 人, 小鼠 FACS, IF, IHC (f) 宿主: 小鼠 Monoclonal DF1485 unconjugated
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- 应用备注
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Immunohistology formalin-fixed 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
- 说明
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DU145 cells. Tonsil, lymph node or breast carcinomas.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS (no BSA, no azide)
- 储存液
- Azide free
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