N-Cadherin 抗体 (Intracellular) (CF®740)
Quick Overview for N-Cadherin 抗体 (Intracellular) (CF®740) (ABIN7650243)
抗原
See all N-Cadherin (CDH2) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- Intracellular
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原理
- N-Cadherin / Cadherin-2 / CD325 (NCAD) (CDH2/1573), CF740 conjugate
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产品特性
- This antibody recognizes a protein of ~140 kDa, identified as N-Cadherin (NCAD), also known as CD325. N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. Expression of N-cadherin has been reported on a variety of normal tissues including neuronal, endothelial and muscle cells, and a subpopulation of early hematopoietic progenitor cells. Results aid in the classification of malignant non-carcinomatous neoplasms including mesotheliomas, chordomas, synovial sarcomas, malignant melanomas, epithelioid sarcomas, epithelioid angiosarcomas, clear cell sarcomas as well as serous and endometrioid tumors of the ovary have been demonstrated to be N-cadherin positive, whereas mucinous tumors are negative. Other N-cadherin-positive neoplasms include renal cell carcinomas and some variant breast tumors, including medullary breast carcinomas and sarcomatoid metaplastic breast carcinomas. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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免疫原
- Recombinant human CDH2 intracellular domain
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亚型
- IgG1, kappa
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anti-Cadherin 2 (CDH2) (AA 701-800) antibody
CDH2 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 犬, 猴 WB, IHC, FACS, IHC (p) 宿主: 小鼠 Monoclonal 2G7 unconjugated
anti-Cadherin 2 (CDH2) (AA 750-850) antibodyCDH2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 5D5 unconjugated
anti-Cadherin 2 (CDH2) (AA 45-238) antibodyCDH2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 685-784) antibodyCDH2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (AA 295-414) antibodyCDH2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 2 (CDH2) (Extracellular Domain) antibodyCDH2 适用: 人 WB, IHC, IF, FACS, IHC (f) 宿主: 小鼠 Monoclonal 8C11 unconjugated
anti-Cadherin 2 (CDH2) antibodyCDH2 适用: 人 WB, IHC, IHC (f) 宿主: 兔 Monoclonal unconjugated Recombinant Antibody
anti-Cadherin 2 (CDH2) (AA 701-714), (Middle Region) antibodyCDH2 适用: 人, 小鼠, 大鼠 WB, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
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应用备注
- Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody. Immunofluorescence: 1-2 μg/mL. Immunohistology (formalin): 0.5-1 μg/mL. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min. Flow Cytometry 0.5-1 μg/million cells/0.1 mL. Western blotting 0.5-1 μg/mL. Optimal dilution for a specific application should be determined by user
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说明
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Positive Control: HeLa or HUVEC cells. Heart, Pancreas or Cerebral Cortex
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 mg/mL
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缓冲液
- PBS, 0.1 % rBSA, 0.05 % azide
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Protect from light
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储存条件
- 4 °C
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储存方法
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Stable at room temperature or 37°C for 7 days.
Protect from light
Store at 2 to 8°C. Protect fluorescent conjugates from light
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有效期
- 24 months
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- N-Cadherin (CDH2) (Cadherin 2 (CDH2))
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别名
- Cadherin-2
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背景
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Synonyms: Cadherin-2 N cadherin neuronal, Cadherin-2 type 1, Cadherin-2, Calcium dependent adhesion protein neuronal, CD325, CDH2, CDHN, CDw325, N-Cadherin, NCAD
Gene Symbol: CDH2
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分子量
- 130-140 kDa
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基因ID
- 1000
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UniProt
- P19022
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途径
- Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane
抗原
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