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PUMA 抗体 (AA 131-180)

Name: PUMA 抗体 (AA 131-180)
SKU: ABIN731528
Availability: OutOfStock

This anti-PUMA antibody is a 兔多克隆 antibody detecting PUMA in WB, ELISA, IP, FACS, IHC (p), IF (cc), IF (p) 和 IHC (fro). Suitable for 人, 大鼠和 Pig. This Primary Antibody has been cited in 3+ publications.

Erianin induced mitochondrial apoptosis in liver cancer cells. (PUMA 抗体 (AA 131-180))

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (ABIN731528) at 1:200 followed by conjugation to the secondary antibody and DAB staining (PUMA 抗体 (AA 131-180))

IHC

K562 lysates probed with PUMA Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C. (PUMA 抗体 (AA 131-180))

U87MG cells probed with Puma Polyclonal Antibody, Unconjugated at 1:50 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange). (PUMA 抗体 (AA 131-180))

FACS

4 images

(3 references)

产品编号 ABIN731528

发货至: 中国

Quick Overview for PUMA 抗体 (AA 131-180) (ABIN731528)

抗原

See all PUMA (BBC3) 抗体

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

适用

人, 大鼠, Pig

宿主

兔

克隆类型

多克隆

标记

This PUMA antibody is un-conjugated

应用范围

Western Blotting (WB), ELISA, Immunoprecipitation (IP), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

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AA 131-180

交叉反应

人, Pig, 大鼠

预测反应

Mouse,Dog,Cow

纯化方法

Purified by Protein A.

免疫原

KLH conjugated synthetic peptide derived from human PUMA

亚型

IgG
anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 119-154) antibody
BBC3 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB01353 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (BH3 Domain), (C-Term) antibody
BBC3 适用: 人, 小鼠 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 186-199) antibody
BBC3 适用: 人 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 适用: 人, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 98-152) antibody
BBC3 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 5D10 unconjugated

anti-BCL2 Binding Component 3 (BBC3) (C-Term) antibody
BBC3 适用: 人, 小鼠 WB, IHC, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 2-16) antibody
BBC3 适用: 人, 大鼠, 小鼠 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (AA 109-137) antibody
BBC3 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-BCL2 Binding Component 3 (BBC3) (N-Term) antibody
BBC3 适用: 人 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

应用备注

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
IP(1-2 μg)

限制

仅限研究用
状态

Liquid

浓度

1 μg/μL

缓冲液

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

有效期

12 months
Su, Shi, Yan, Xi, Su: "Anticancer bioactive peptides suppress human colorectal tumor cell growth and induce apoptosis via modulating the PARP-p53-Mcl-1 signaling pathway." in: Acta pharmacologica Sinica, Vol. 36, Issue 12, pp. 1514-9, (2016) (PubMed).

Qiu, Cai, Gao, Gu, Liu: "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." in: Cancer letters, Vol. 356, Issue 2 Pt B, pp. 910-7, (2014) (PubMed).

Zhang, Liu, Xia, Zhang, Zhang, Zhao, Chao, Jiang, Jiang: "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." in: International journal of molecular medicine, Vol. 33, Issue 1, pp. 151-9, (2013) (PubMed).
抗原

PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

别名

PUMA

背景

Synonyms: JFY1, PUMA, JFY-1, Bcl-2-binding component 3, p53 up-regulated modulator of apoptosis, BBC3
Background: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity.

基因ID

27113

UniProt

Q96PG8

途径

p53 Pathway, Positive Regulation of Endopeptidase Activity